发明名称 |
Self-calibrating gradient dilution in a constitutent assay and gradient dilution apparatus performed in a thin film sample |
摘要 |
A method and apparatus for measuring antibody titers in a thin film sample in an automated system which does not require multiple dilutions. The system provides a simple method for creating an in-situ dilution within a sample analysis chamber without the use of any precision fluid-handling components, and further, to use the same principles to provide a wide range of sample dilutions within the chamber so as to obviate the need for additional dilution steps when dealing with samples possibly containing wide ranges of analyte concentrations. |
申请公布号 |
US9274094(B2) |
申请公布日期 |
2016.03.01 |
申请号 |
US201213550131 |
申请日期 |
2012.07.16 |
申请人 |
Abbott Point of Care, Inc. |
发明人 |
Wardlaw Stephen C.;Levine Robert A. |
分类号 |
G01N33/53;G01N33/48;G01N33/543;G01N21/64 |
主分类号 |
G01N33/53 |
代理机构 |
O'Shea Getz P.C. |
代理人 |
O'Shea Getz P.C. |
主权项 |
1. A method for determining the relative dilution of a solution containing a ligand which is directed against a target analyte, and a liquid sample containing the target analyte to be analyzed, which target analyte sample is miscible with said ligand solution, at least one of said solution and said sample containing a detectable sensible marker, said method comprising the steps of:
a) placing a first amount of said solution in a first area of a planar reaction chamber having a thin through thickness dimension; b) placing a second amount of said target analyte sample in a second area of the chamber, which second area is adjacent the first area of said planar chamber, and which second amount is less than the first amount; c) allowing or causing said solution and said sample to admix with each other until an even solution-sample mixture is created in said chamber; d) electronically imaging or scanning said resultant solution-sample mixture in said chamber to determine sensible marker signal; and e) electronically calculating a relative concentration or dilution of each of said solution and sample from any imaged area by measuring the concentration of any marker in said mixture in said imaged area. |
地址 |
Princeton NJ US |