Nucleic acid detection method, and device and kit for use in same

摘要

The present invention aims to solve problems in the analysis of amplified nucleic acids, i.e., cost, workability, and contamination and pollution of samples. In the present invention, a sample containing a nucleic acid and a reagent for detecting the nucleic acid are mixed in a closed system. In this regard, the nucleic acid is amplified in the same closed system, and then mixed with the detecting reagent without opening the system. In order to carry out this method, for example, a device that includes the detecting reagent shielded by a coating material or the like is used.

主权项

1. A method of detecting a nucleic acid, comprising the steps of:
contacting a nucleic acid with a detecting reagent containing a leuco dye in a closed system; wherein the leuco dye is initially colorless, and wherein the nucleic acid reacts as a color developer with the colorless leuco dye to produce a colored dye; and observing a color change of at least one of the nucleic acid and the detecting reagent under visible light.