| 摘要 |
The invention relates to bioengineering, in particular to producing endothelial cells from human embryonal stem cells and to the use thereof. The inventive method for producing endothelial cells from human embryonal stem cells involves differentiating human embryonal stem cells on a substrate and in a synthetic medium which provide the oriented direct differentiation of human embryonal stem cells into endothelial cells based on the mixture of DMEM/FI2 and a KO serum substitute having VEGF, SCF, BMP4, bFGF and TGFbeta growth factors and taken in effective quantities, wherein TGFbeta is added on 3rd-6th day of differentiation. A differentiation medium based on the mixture of DMEM/FI2 with fetal bovine serum having VEGF, SCF and bFGF growth factors can be also used. The separation is carried out on 3rd-9th day according to an immunological selection method using markers specific for endothelial cells and, afterwards, the thus produced endothelial cells are cultivated in the above-mentioned medium with an inoculum density which is equal to or greater than 50000 cells per 1 cm. |
