TWO-DIMENSIONAL LIQUID CHROMATOGRAPHY SYSTEM FOR HEART-CUT METHOD

摘要

Provided is a two-dimensional liquid chromatography system, and more particularly, a two-dimensional liquid chromatography system capable of performing both independent one-dimensional separation through a reversed-phase or normal-phase chromatography method and two-dimensional separation for removing a matrix effect in a single system.

主权项

1. A two-dimensional liquid chromatography system characterized in that in a reversed-phase or normal-phase two-dimensional liquid chromatography system, a first switching valve and a second switching valve are switched so that among materials separated in a first column, only a material of interest is selectively re-separated in a second column supposing that the material of interest is co-eluted with the matrix during the separation of materials in the first column, but the material of interest reaching the second column is diluted by a diluting mobile phase supplied through a second mobile-phase pump connected to the first switching valve to thereby be analyzed in a mass spectrometer (MS).