Seed-preferred promoter from peroxiredoxin gene from flax

摘要

The present invention relates to expression cassettes comprising transcription regulating nucleotide sequences with seed-preferential or seed-specific expression profiles in plants obtainable from the Arabidopsis thaliana gene described by the locus At1g48130 or its orthologs from other plant species (such as Linum usitatissimum). The transcription regulating nucleotide sequences preferably exhibit strong expression activity especially in seeds and seed tissues.

主权项

1. A method for directing expression of at least one nucleic acid sequence in early to mature rapeseed embryos, said method comprising:
i) introducing into a rapeseed plant or plant cell an expression cassette for regulating expression of at least one nucleic acid sequence in early to mature plant embryos; ii) selecting a transgenic rapeseed plant or plant cell comprising said expression cassette; and iii) regenerating a rapeseed plant from the transgenic rapeseed plant cell, wherein said expression cassette comprises at least one transcription regulating nucleotide sequence, and operably linked thereto at least one nucleic acid sequence which is heterologous in relation to said transcription regulating nucleotide sequence, and wherein said at least one transcription regulating nucleotide sequence directs expression of the operably linked at least one nucleic acid sequence in early to mature embryos of said transgenic rapeseed plant or said rapeseed plant and comprises the nucleotide sequence of SEQ ID NO: 9, 10, or 11.