Amino acid sequence presenting fusion polypeptide and its use

摘要

Herein is reported a fusion polypeptide according to formula (I): NH2—S2—X1—S1—COOH, wherein X1 comprises either a random amino acid sequence or an amino acid sequence derived from a first polypeptide, S2 and S1 are non-overlapping amino acid sequences derived from a second polypeptide, and denotes a peptide bond, wherein the second polypeptide is a polypeptide with peptidyl-prolyl cis/trans-isomerase activity (PPIase activity) or is derived from the FKBP-fold domain family, wherein X1 is inserted in place of the insert-in-flap-domain of the second polypeptide.

主权项

1. A method for eliciting an immune response in an experimental animal against a polypeptide said method comprising: administering to the experimental animal a fusion polypeptide for at least one time, said fusion polypeptide comprising a polypeptide according to formula I:
NH2—S2—X1—S1—COOH  (formula I), wherein: X1 comprises a random amino acid sequence or an amino acid sequence derived from a first polypeptide, S2 and S1 are non-overlapping amino acid sequences derived from a second polypeptide, and
denotes a peptide bond, wherein the second polypeptide is selected from the group consisting of human FKBP12, Arabidopsis thaliana FKBP13, Thermus thermophilus SlyD, Escherichia coli SlyD and Thermococcus gammatolerans SlyD, and wherein X1 is inserted in place of the insert-in-flap-domain (IF-domain) of the second polypeptide, and wherein X1 is the amino acid sequence of the polypeptide.