Electrophoresis buffer for faster migration, improved resolution and extended shelf-life

摘要

There is provided an electrolyte solution for extending shelf life, and/or accelerating or improving resolution or improving transfer efficacy for blot applications, or accelerating and improving resolution, or accelerating and improving transfer efficacy of gel electrophoresis and containing Tris(hydroxymethyl)aminomethane (TRIS), at least one zwitterion, and water. The electrolyte solution may be used in buffer systems for gel electrophoresis and the preparation of gels for gel electrophoresis, such as Western blot.

主权项

1. An electrolyte solution comprising:
Tris(hydroxymethyl)aminomethane (TRIS); at least one zwitterion chosen from 2-amino-2methyl-1,3-propanediol (AMPD), N,N-bis[2-hydroxyethyl]-2-aminoethanesulphonic acid (BES), N-Glycylglycine (Gly-Gly), 4-(2-hydroxyethyl)piperazine-1-propanesulfonic acid (EPPS or HEPPS), and 3-(cyclohexylamino)-2-hydroxy-1-propanesulfonic acid (CAPSO), N-(1,1-Dimethyl-2-hydroxyethyl)-3-amino-2-hydroxypropanesulfonic acid (AMPSO), 3-(cyclohexylamino)-1-propanesulfonic acid (CAPS), 2-(cyclohexylamino)ethanesulfonic acid (CHES), 3-[N-Bis(hydroxyethyl)amino]-2-hydroxypropanesulfonic acid (DIPSO), N-(2-Hydroxyethyl)piperazine-N′-(4-butanesulfonic acid) (HEPBS), 4-(N-Morpholino)butanesulfonic acid (MOBS), piperazie-N,N′-bis[2-hydroxypropanesulphonic]acid (POPSO), N-Tris(hydroxymethyl)methyl-4-aminobutanesulfonic acid (TABS), N-Tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid (TAPS), (2-[2-hydroxy-1,1-bis(hydroxymethyl)ethylamino]ethanesulphonic acid) (TES), (2-[2-acetamino]-2-aminoethanesulphonic acid) (ACES), (1,4-piperazinediethanesulphonic acid) (PIPES), and Piperazine-N,N′-bis(3-propanesulfonic Acid) (PIPPS); water, and at least one of methanol, 0.5% (wt/vol) or less sodium dodecyl sulphate (SDS), or methanol and 0.5% (wt/vol) or less sodium dodecyl sulphate (SDS); wherein the pH of the electrolyte solution is from about 8.0 to about 8.8, wherein said electrolyte solution is for at least one of: a shorter electrophoresis time of a gel electrophoresis, an improved resolution of a gel electrophoresis, an improved transfer efficacy of a gel electrophoresis, and a shorter transfer time of a gel electrophoresis, compared to a Tris-Glycine-SDS electrolyte solution at pH 8.3.