3-D Localization And Imaging of Dense Arrays of Particles

摘要

Systems, methods, and computer program products are disclosed to localize and/or image a dense array of particles. In some embodiments, a plurality of particles may be imaged using an imaging device. A plurality of point spread function dictionary coefficients of the image may be estimated using a point spread function dictionary; where the point spread function dictionary can include a plurality of spread function responses corresponding to different particle positions. From the point spread function dictionary coefficients the number of particles in the image can be determined. Moreover location of each particle in the image can be determined from the point spread function dictionary co efficients.

主权项

1. A method for detecting the number and/or location particles comprising:
imaging a plurality of particles using an imaging system having a point spread function, wherein the imaging returns an image; estimating a plurality of point spread function dictionary coefficients of the image using a point spread function dictionary, wherein the point spread function dictionary includes a plurality of point spread function responses that each correspond with a different particle position; and determining the number and/or location of the particles in the image from the point spread function dictionary coefficients.