METHODS AND APPARATUS FOR MONITORING INTERACTIONS BETWEEN PARTICLES AND MOLECULES USING NANOPHOTONIC TRAPPING

摘要

A method for characterizing an interaction between a first particle and a second particle is provided. The method includes the steps of: (i) providing an optical trap system including a photonics-based trap, a light source, and a camera; (ii) optically trapping, using the photonics-based trap, the first particle; (iii) obtaining a first measurement of a trap stiffness of the photonics-based trap; (iv) introducing the second particle to the optically trapped particle; (v) incubating the first and second particles under conditions suitable for an interaction between the first and second particles; (vi) obtaining a second measurement of the trap stiffness of the photonics-based trap after the incubation; and (vii) determining, using the first measurement of trap stiffness and the second measurement of trap stiffness, a property of the interaction between the first particle and the second particle.

主权项

1. A method for characterizing an interaction between a first particle and a second particle, the method comprising the steps of:
providing an optical trap system, the optical trap system comprising a photonics-based trap, a light source, and a camera; optically trapping, using the photonics-based trap, the first particle; obtaining a first measurement of a trap stiffness of the photonics-based trap; introducing the second particle to the optically trapped particle; incubating the first and second particles under conditions suitable for an interaction between said first and second particles; obtaining a second measurement of the trap stiffness of the photonics-based trap after the incubation; and determining, using the first measurement of trap stiffness and the second measurement of trap stiffness, a property of the interaction between the first particle and the second particle.