Analyte detection

摘要

The present disclosure provides methods and/or kits for detecting an analyte in a sample. Some embodiments provide a method for detecting a non-nucleic acid analyte in a sample using a solid substrate comprising a bound immobilization agent and an antibody capture agent and a detectable agent, which can bind to the analyte. The antibody capture agent comprises, at a plurality of sites, a ligand for the immobilization agent. A complex between the analyte, the antibody capture agent and a detectable agent is formed and immobilized on the solid substrate by binding between the immobilization agent and the ligand. In some embodiments, the ligand and the immobilization agent are a binding pair comprising a peptide tag and an anti-peptide tag antibody.

主权项

1. A method for detecting an analyte in a sample, comprising:
i) forming an analyte complex comprising the analyte bound to a tagged antibody capture agent and separately to a detectable agent, wherein the tagged antibody capture agent comprises a plurality of covalently conjugated peptide tags; ii) introducing the analyte complex to an anti-peptide antibody immobilized on a substrate; iii) immobilizing the analyte complex to the substrate by binding at least one of the plurality of peptide tags with the anti-peptide antibody; and iv) detecting the analyte by detection of the detectable agent.