Method of inducing an immune response

摘要

A method is provided for inducing or enhancing an immune response in a mammal to a target polypeptide expressed in a plurality of cells of the mammal, which method comprises administering to the mammal an inhibitory nucleic acid which targets a region of a ribonucleic acid (RNA) which encodes said polypeptide. Also provided is a pharmaceutical composition comprising an inhibitory nucleic acid which targets a region of an RNA which encodes a target polypeptide expressed in a plurality of cells of a mammal, such that translation of an aberrant form of the target polypeptide occurs in said cells, said truncated form of the target polypeptide comprising one or more T cell epitopes; together with a pharmaceutically acceptable carrier or diluent.

主权项

1. A method of inducing or enhancing an immune response in a mammal to an E6/E7 polypeptide of HPV, comprising administering to the mammal an shRNA which targets an E6/E7 region of an HPV RNA consisting of nucleotides 181-198 of SEQ ID NO: 3, at least a portion of the E6/E7 polypeptide comprising a T cell epitope and presented on a surface of cells bound to major histocompatibility complex (MHC), the shRNA comprising an antisense strand consisting of a sequence complementary to nucleotides 181-198 of SEQ ID NO: 3, and wherein the administered shRNA is insufficient to kill directly cells expressing the E6/E7 polypeptide.