Methods for the Detection of DNA-RNA Proximity in Vivo

摘要

Disclosed is a method for detecting spatial proximity relationships between RNA and DNA molecules in a cell. The method includes: providing a sample of RNA and DNA wherein the RNA and fDNA have ends capable of joining to other DNA and RNA, respectively; joining at least one end of the fragmented RNA to the end of at least one fragmented DNA, to create at least one joined RNA-DNA hybrid molecule, wherein the join encodes the information about the proximity of the RNA and DNA in the cell; reverse transcribing the at least one joined rRNA-DNA hybrid molecule to create least one target join DNA molecule that retains the information of the join, and determining the sequence of the target join thereby detecting spatial proximity relationships between RNA and DNA molecules in a cell.

主权项

1. A method for detecting spatial proximity relationships between RNA and DNA molecules in a sample, comprising:
providing a sample comprising fragmented RNA and DNA, wherein the RNA and DNA have ends capable of joining to other fragmented DNA and RNA, respectively; joining at least one end of the fragmented RNA to the end of at least one fragmented DNA, to create at least one joined RNA-DNA hybrid molecule, wherein the join encodes the information about the proximity of the RNA and DNA in the sample; reverse transcribing the at least one joined RNA-DNA hybrid molecule to create least one target join DNA molecule that retains the information of the join; and determining the sequence of the at least one target join DNA molecule, thereby detecting spatial proximity relationships between RNA and DNA molecules.