发明名称 |
ヘモグロビンA1c測定法およびそれに用いる酵素とその製造法 |
摘要 |
There is provided a method for specifically determining a glycated ²-chain N-terminal of glycated hemoglobin using enzymes without a separation operation, and a determination reagent kit therefor. A protease that cleaves a glycated amino acid and/or a glycated peptide from a glycated ²-chain N-terminal without substantially cleaving a glycated amino acid or a glycated peptide from a glycated ±-chain N-terminal of glycated hemoglobin or a fragment thereof is screened. The method of specifically determining a glycated ²-chain N-terminal of glycated hemoglobin and the determination reagent kit are provided by using the protease obtained by the screening method. According to the present invention, a glycated ²-chain N-terminal of glycated hemoglobin can specifically be determined without a separation operation. |
申请公布号 |
JP5855592(B2) |
申请公布日期 |
2016.02.09 |
申请号 |
JP20130022996 |
申请日期 |
2013.02.08 |
申请人 |
旭化成ファーマ株式会社;イチビキ株式会社 |
发明人 |
松岡 毅;古賀 晋治;高妻 卓司;芳陵 一生;西村 篤寿;伊藤 誉;熊澤 利彦;畔柳 孝 |
分类号 |
C12Q1/26;C12N1/21;C12N9/02;C12N9/50;C12N15/53;C12Q1/37;G01N33/66;G01N33/72 |
主分类号 |
C12Q1/26 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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