Method of producing erythrocytes without feeder cells

摘要

Provided herein are methods of producing erythrocytes from hematopoietic cells, particularly hematopoietic cells from placental perfusate in combination with hematopoietic cells from umbilical cord blood, wherein the method results in accelerated expansion and differentiation of the hematopoietic cells to more efficiently produce administrable erythrocytes. Further provided herein is a bioreactor in which hematopoietic cell expansion and differentiation takes place.

主权项

1. A medium for growth or differentiation of hematopoietic cells comprising SCF at a concentration of about 10 to about 10,000 ng/mL, IL-3 at a concentration of about 0.01 to about 500 ng/mL, and EPO at a concentration of about 0.1 to about 10 IU/mL,
wherein said medium comprises insulin-like growth factor 1 (IGF-1) at a concentration of about 1 to about 1000 ng/mL, wherein said composition does not comprise one or more of Flt-3L, IL-11, thrombopoietin (Tpo), homeobox-B4 (HoxB4), or methylcellulose, wherein said SCF, IL-3 and Epo are not comprised within an undefined component of said medium, and wherein said medium further comprises lipids at a concentration of about 1 to about 1000 μg/mL, wherein said lipids comprise a mixture of protein and cholesterol.