Amphiphilic block copolymers for nucleic acid delivery

摘要

The present invention provides a composition comprising vesicles and encapsulated within the vesicles, nucleic acid comprising less than 1000 nucleotides, wherein the vesicles comprise an amphiphilic block copolymer having a hydrophilic and a hydrophobic block.;Methods of forming vesicles and methods of delivering nucleic acid, in particular, iRNA into cells, are also provided.

主权项

1. A composition comprising nanovesicles and encapsulated within the aqueous core of the nanovesicles, nucleic acid comprising less than 1000 nucleotides, wherein the nanovesicles comprise an amphiphilic block copolymer having a hydrophilic block formed from 2-methacryloyloxy ethyl phosphorylcholine and a hydrophobic block formed from 2-(diisopropyl)amino ethyl methacrylate, wherein the degree of polymerisation of the hydrophilic block is about 20 to 25 and the degree of polymerisation of the hydrophobic block is about 70 to 75 and wherein the nucleic acid is siRNA.