| 发明名称 | PmST1 mutants for chemoenzymatic synthesis of sialyl lewis X compounds | ||
| 摘要 | The present invention provides mutants of PmST1 for the preparation of sialyl-Lewisx oligosaccharides, and other sialosides with decreased sialidase activity. | ||
| 申请公布号 | US9255257(B2) | 申请公布日期 | 2016.02.09 |
| 申请号 | US201214237334 | 申请日期 | 2012.08.06 |
| 申请人 | THE REGENTS OF THE UNIVERSITY OF CALIFORNIA | 发明人 | Chen Xi;Sugiarto Go;Lau Kam |
| 分类号 | C12P19/18;C12N9/10 | 主分类号 | C12P19/18 |
| 代理机构 | Kilpatrick Townsend and Stockton LLP | 代理人 | Kilpatrick Townsend and Stockton LLP |
| 主权项 | 1. An isolated glycosyltransferase, wherein the amino acid corresponding to position 120 of SEQ ID NO:1 is any amino acid other than M, the amino acid corresponding to position 289 of SEQ ID NO:1 is any amino acid other than R, wherein the glycosyltransferase has decreased α2-3 sialidase or donor substrate hydrolysis activity compared to a control glycosyltransferase, wherein the amino acid of the glycosyltransferase corresponding to position 120 of SEQ ID NO:1 is M, the amino acid corresponding to position 247 of SEQ ID NO:1 is E, and the amino acid corresponding to position 289 of SEQ ID NO:1 is R, and wherein the glycosyltransferase is a member of the glycosyltransferase family 80 (GT80). | ||
| 地址 | Oakland CA US | ||