RANDOM RNAi LIBRARIES, METHODS OF GENERATING SAME, AND SCREENING METHODS UTILIZING SAME

摘要

This invention provides expression vectors for a ribonucleic acid (RNA) molecule comprising a double-stranded region of random sequence, sets and libraries of same, methods of generating same, and methods for identifying an RNA therapeutic or RNA molecule that has an ability to affect a biological parameter, for identifying a drug target for a disease or disorder of interest, and for identifying a variant of an RNA molecule that has an altered ability to affect a biological parameter of interest.

主权项

1. A set or library of recombinant expression vectors, wherein said set or library of recombinant expression vectors expresses a set or library of ribonucleic acid (RNA) molecules, wherein each RNA molecule comprises:
(a) a first region, wherein either (i) the sequence of said first region is substantially random; or (ii) said first region comprises a first sub-region of substantially random sequence and a second sub-region common to said set or library of recombinant expression vectors; (b) a second, non self-complementary region; (c) a third region substantially complementary to said first region, wherein said second, non self-complementary region is common to said set or library of recombinant expression vectors.