Detection and Classification of an Anticoagulant Using A Clotting Assay

摘要

In some embodiments, the invention provides methods for detecting and/or classifying an anticoagulant at a therapeutically relevant amount or higher in a patient, including subjecting a sample of a control blood component (known not to contain the anticoagulant) to a clotting assay in the presence of a Factor Xa reagent to obtain a control clotting measurement; and subjecting a sample of a blood component from a patient suspected of having the anticoagulant to the clotting assay in the presence of the Factor Xa reagent to obtain a patient clotting measurement, wherein the patient clotting measurement sample greater than the control clotting measurement indicates the presence of the anticoagulant at a therapeutically relevant amount or higher in the patient. In some embodiments, the invention includes methods for classifying an anticoagulant as an anti-Factor Xa or a direct thrombin inhibitor anticoagulant using a clotting assay in the presence of an ecarin reagent.

主权项

1. A method for detecting an anticoagulant at a therapeutically relevant amount or higher in a patient suspected of having an anticoagulant, the method comprising
a) subjecting a sample of a control blood component known not to contain the anticoagulant, to a clotting assay in the presence of a Factor Xa reagent to obtain a control Factor Xa clotting measurement; and b) subjecting a sample of a blood component from the patient suspected of having the anticoagulant to the clotting assay in the presence of the Factor Xa reagent to obtain a patient Factor Xa clotting measurement, wherein the patient Factor Xa clotting measurement greater than the control Factor Xa clotting measurement indicates the presence of the anticoagulant at a therapeutically relevant amount in the patient and wherein the patient Factor Xa clotting measurement less than or equal to the control Factor Xa clotting measurement indicates the absence of the anticoagulant at a therapeutically relevant amount or higher in the patient.