发明名称 |
Analyte Detection Enhancement by Targeted Immobilization, Surface Amplification, and Pixelated Reading and Analysis |
摘要 |
This disclosure provides, among other things, a method of sample analysis, that comprises: (a) binding target analytes to capture agents that are attached to a surface of a plate, wherein the plate comprises (i) a sensing amplification layer comprises nanostructures that enhance signals and (ii) the capture agents are attached to said sensing amplification layer; (b) reading the plate with a reading device to produce an image of signals that represent individual binding events; and (c) identifying and counting individual binding events in an area of the image, thereby providing an estimate of the amount of one or more analytes in the sample. A system for performing the method is also provided. |
申请公布号 |
US2016033496(A1) |
申请公布日期 |
2016.02.04 |
申请号 |
US201414775638 |
申请日期 |
2014.03.14 |
申请人 |
THE TRUSTEES OF THE PRINCETON UNIVERSITY |
发明人 |
Chou Stephen Y.;ZHOU Liang-Cheng |
分类号 |
G01N33/543;G01N33/574;G02B21/00 |
主分类号 |
G01N33/543 |
代理机构 |
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代理人 |
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主权项 |
1. A method of sample analysis, comprising:
(a) binding target analytes to capture agents that are attached to a surface of a plate, wherein the plate comprises (i) a sensing amplification layer comprises nanostructures that enhance signals and (ii) the capture agents are attached to said sensing amplification layer; (b) reading the plate with a reading device to produce an image of signals that represent individual binding events; and (c) identifying and counting individual binding events in an area of the image, thereby providing an estimate of the amount of one or more analytes in the sample. |
地址 |
Princeton NJ US |