| 发明名称 |
RAPID REAL TIME MULTIPOINT PROCEDURE FOR OPTIMIZING SPERM STATE FOR USE IN ASSISTED REPRODUCTIVE TECHNOLOGIES |
| 摘要 |
A method for adjusting the maturation state of mammalian sperm for use in assisted reproductive technologies (ART) is disclosed. A mammalian ejaculate is provided and incubated under controlled conditions. Aliquots of the ejaculate are assayed during incubation period at intervals to determine maturation state and changes in the maturation state by observing the percent positive cells in the aliquot. The assays are repeated with successive aliquots at intervals during incubation to observe real time changes in the maturation state. The ejaculate remaining is processed for the desired ART after the percentage of positive cells in the latest aliquot being assayed begins to decline. |
| 申请公布号 |
US2016033483(A1) |
申请公布日期 |
2016.02.04 |
| 申请号 |
US201414777407 |
申请日期 |
2014.03.11 |
| 申请人 |
COHEN Barb Ariel |
发明人 |
Cohen Barb A. |
| 分类号 |
G01N33/50;A01N1/02;G01N33/569 |
主分类号 |
G01N33/50 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for adjusting the maturation state of mammalian sperm for use in an assisted reproductive technology (ART), said method comprising:
providing a mammalian ejaculate; incubating the ejaculate under controlled conditions; assaying an aliquot of the ejaculate during incubation period to determine maturation state by observing the percent positive cells in the aliquot; repeating the assaying step with successive aliquots at intervals during incubation to observe real time changes in the maturation state; and processing the ejaculate remaining for the desired ART after the percentage of positive cells in the latest aliquot being assayed begins to decline. |
| 地址 |
Watertown MA US |
