NOVEL METHOD AND DEVICE FOR WHOLE-CELL BACTERIAL BIO-CAPACITOR CHIP FOR DETECTING CELLULAR STRESS INDUCED BY TOXIC CHEMICALS

摘要

The present invention is directed to methods and a bio-capacitor sensing device for the detection of toxic chemicals using bacteria. The sensing platform comprises gold interdigitated capacitor with a defined geometry, a layer of carboxy-CNTs immobilized with viable E. coli cells as sensing elements. Also included are methods of making the bio-capacitor device and methods for detecting toxic chemicals that induce cellular stress response. The present innovation discloses the development of a bio capacitor chips immobilized with carboxy-CNTs tethered E. coli bacteria. In addition, the present invention also includes determination of behavior and characteristics of chemically stimulated bacteria on biochip using electric field including frequency and/or amplitude as controlling parameters.

主权项

1. A method of producing a bio-capacitor sensing device, the method comprising the steps of:
a) providing a substrate; b) depositing a metal layer on the substrate to form a capacitor, wherein the metal layer comprises at least one electrode in interdigitated structure; c) patterning the metal layer on the capacitor; d) covalently attaching a layer of carboxylated carbon nanotubes (carboxy-CNTs) to the capacitor to form a carboxy-CNT activated capacitor; e) immobilizing viable cells to the carboxy-CNT activated capacitor, whereby the viable cells are sensing elements that are capable of adapting to respond with a target chemical, wherein the viable cells are monitored for stress imposed by the target chemical on the viable cells.