Production of high levels of DHA in microalgae using modified amounts of chloride and potassium

摘要

Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.

主权项

1. A method of producing a biomass comprising docosahexaenoic acid (DHA), comprising:
culturing a heterotrophic microalga in a culture medium to produce said biomass, wherein the culture medium comprises: chloride ions at a concentration of less than or equal to 2 g/L; potassium ions at a concentration of greater than or equal to 0.5 g/L; and a pH less than or equal to 4.5; and recovering the DHA-containing biomass from the microalga.