SEQUENCE SPECIFIC ANTIMICROBIALS

摘要

Provided are compositions and methods for selectively reducing the amount of antibiotic resistant and/or virulent bacteria in a mixed bacteria population, or for reducing any other type of unwanted bacteria in a mixed bacteria population. The compositions and methods involve targeting bacteria that are differentiated from other members of the population by at least one unique clustered regularly interspaced short palindromic repeats (CRISPR) targeted DNA sequence. The compositions and methods can be readily adapted to target any bacteria or any bacteria plasmid, or both.

主权项

1. A pharmaceutical composition for selectively reducing the amount of antibiotic resistant and/or virulent Staphylococcus aureus (S. aureus) bacteria in a mixed bacterial population, the composition comprising a pharmaceutically acceptable carrier and a packaged, recombinant phagemid, wherein the phagemid comprises a clustered regularly interspaced short palindromic repeats (CRISPR) system, wherein the CRISPR system comprises nucleotide sequences encoding:
i) a CRISPR-associated (Cas) 9 enzyme; and ii) a targeting RNA selected from:
a) at least one S. aureus chromosome targeting RNA;b) at least one S. aureus plasmid targeting RNA; andc) a combination of a) and b).