Three-Dimensional Image Processing to Locate Nanoparticles in Biological and Nonbiological Media

摘要

Disclosed are various embodiments for methods and systems for three-dimensional imaging of subject particles in media through use of dark-field microscopy. Some examples, among others, include a method for obtaining a three-dimensional (3D) volume image of a sample, a method for determining a 3D location of at least one subject particle within a sample, a method for determining at least one spatial correlation between a location of at least one subject particle and a location of at least one cell structure within a cell and/or other similar biological or nonbiological structure, a method of displaying a location of at least one subject particle, method for increasing the dynamic range of a 3D image acquired from samples containing weak and strong sources of light, and method for sharpening a 3D image in a vertical direction.

主权项

1. A method for obtaining a three-dimensional volume image of a sample, the method comprising:
obtaining, by dark-field microscopy, a plurality of two-dimensional images from the sample, the plurality of images comprising at least one two-dimensional image taken at each of a plurality of equally spaced sample locations along a direction of focus, each two-dimensional image containing both in-focus and out-of-focus light from the sample; inputting the plurality of two-dimensional images to a three-dimensional computational method for determining a location of at least one structure present in the sample; and formulating a three-dimensional volume image of the sample, the three-dimensional image showing the location of the at least one structure present in the sample.