A Biosensor Device to Target Analytes in Situ, in Vivo, and/or in Real Time, and Methods of Making and Using the Same

摘要

A biosensor device for the real-time detection of a target analyte includes a receptor component operatively connected to a transducer component which is adapted to interpret and transmit a detectable signal. The receptor component includes a sensing element capable of detecting and binding to at least one target analyte, and a self-assembled monolayer (SAM) layer. The SAM layer is positioned between and in contact with the sensing element and an electrode such that the sensing element, in the presence of the target analyte, causes a detectable signal capable of being transmitted to the electrode. The transducer component includes the electrode and microprocessor configured to screen noise and to pick up impedance change at a very low frequency range.

主权项

1. A biosensor for detecting the presence of a target analyte in a sample, the biosensor comprising:
a transducer component comprising an electrode operatively connected to a microprocessor, the microprocessor being adapted to receive, process and transmit a signal; and, a receptor component having:
i) a sensing element capable of detecting and binding to at least one target analyte present in a sample, andii) a self-assembled monolayer (SAM) layer, the SAM layer being positioned between and in contact with the sensing element and the electrode; the transducer component and the receptor component being capable of being brought into direct contact with the sample in situ, wherein the sensing element, in the presence of the target analyte present in the sample, causes a detectable signal capable of being transmitted to the electrode via the SAM layer.