| 摘要 |
FIELD: medicine.SUBSTANCE: medication is obtained by method, including cultivation of mesenchymal stromal cells of human adipose tissue (MSC AT) of 2-5 passage in growth medium until concentration of total protein in medium 3-4 mg/ml is achieved. Selection and purification of culture medium and its concentration until initial volume of purified culture medium is reduced 9-11 times are carried out with obtaining sterile concentrate, with its further lyophilisation. Medication contains products of human MSC secretion, including key growth factors: FGF basic in concentration from 2 to 4 pg/ml, HGF in concentration from 40 to 80 pg/ml, VEGF in concentration from 200 to 800 pg/ml, angiopoietin-1 in concentration from 0.5 to 10.0 pg/ml, determined by method of enzyme immunoassay with reduction of lyophilisate in 100 ml of sterile physiological solution. Growth medium contains solution of AdvanceSTEM Cell Culture Media, solution of antibiotics Penicillin-Streptomycin and additives AdvanceSTEM Stem Cell Growth Supplement with the following component ratio, vol %: AdvanceSTEM Cell Culture Media - 85-95, solution of antibiotics Penicillin-Streptomycin - 0.95-1.05, additives to growth medium AdvanceSTEM Stem Cell Growth Supplement - 9.5-10.5. Group of inventions also relate to method of treating burns and wounds.EFFECT: application of claimed group of inventions makes it possible to stimulate healing of burns and wounds due to regenerative action of balanced complex of cytokines and growth factors, secreted by mesenchymal human cells into culture medium.10 cl, 11 dwg, 1 tbl, 10 ex |
