Purified plasmodium and vaccine compositions

摘要

Disclosed are substantially purified Plasmodium sporozoites and preparations of Plasmodium sporozoites substantially separated from attendant non-sporozoite material, where the preparations of Plasmodium sporozoites have increasing levels of purity. Vaccines and pharmaceutical compositions comprising purified Plasmodium sporozoites are likewise provided. Methods of purifying preparations of Plasmodium sporozoites are also provided.

主权项

1. A method of conferring protective immunity in a human against malaria caused by a Plasmodium-species parasite comprising administering to said human host at least one dose of an aseptic, purified preparation of a metabolically active Plasmodium sporozoite which is genetically attenuated by introduction of at least one heritable genetic alteration, and wherein said aseptic, purified preparation is suitable for pharmaceutical use and comprises less than 85 nanograms of attendant material per 25,000 sporozoites, wherein protective immunity is conferred in said human host.