| 发明名称 |
THE TELOMERATOR-A TOOL FOR CHROMOSOME ENGINEERING |
| 摘要 |
The present invention relates to the field of chromosome engineering. More specifically, the present invention provides methods and compositions useful for inducibly linearizing circular DNA molecules in vivo in yeast. In one embodiment, a comprises a nucleic acid encoding a selectable marker, wherein the nucleic acid encoding a selectable marker comprises an intron comprising an endonuclease recognition site flanked by telomere seed sequences. |
| 申请公布号 |
US2016017344(A1) |
申请公布日期 |
2016.01.21 |
| 申请号 |
US201414772832 |
申请日期 |
2014.03.06 |
| 申请人 |
THE JOHNS HOPKINS UNIVERSITY |
发明人 |
Boeke Jef D.;Mitchell Leslie |
| 分类号 |
C12N15/81;C12N15/65 |
主分类号 |
C12N15/81 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A telomerator cassette comprising a nucleic acid encoding a selectable marker, wherein the nucleic acid encoding a selectable marker comprises an intron comprising an endonuclease recognition site flanked by telomere seed sequences. |
| 地址 |
Baltimore MD US |
