ISOLATION OF MEGABASE-SIZED DNA FROM PLANT AND ANIMAL TISSUES

摘要

Disclosed herein are methods for isolation of long DNA molecules, for example megabase-sized genomic DNA molecules, from a biological sample, for example plant and animal tissues.

主权项

1. A method for isolating DNA from a biological sample, the method comprising:
(a) homogenizing the biological sample to generate a homogenate; (b) contacting the homogenate with a DNA and/or protein precipitating agent; (c) embedding the homogenate in a porous matrix; and (d) recovering DNA from the homogenate.