CELL EXPRESSION SYSTEM

摘要

An expression system for expressing a protein comprising: a eukaryotic host cell carrying a dihydrofolate reductase (DHFR) deficiency; and an expression vector, the expression vector encoding the human growth hormone gene; a expression vector, the expression vector comprising: a eukaryotic selectable marker including a minimal SV 40 early promoter driving expression of a sequence encoding dihydrofolate reductase for complementing the DHFR deficiency in the host cell; a prokaryotic selectable marker conveying Ampicillin resistance to a prokaryotic host cell; a prokaryotic Origin of Replication; a plurality of multiple cloning sites (MCS); and at least one protein expression module comprising: a Simian Vacuolating Virus 40 (SV40) early promoter, inclusive of its 72 bp enhancer repeats; and a rabbit β-globin intron sequence being separable from a SV40 p A sequence by a first multiple cloning site, for receiving a coding sequence and expressing a desired protein therefrom.

主权项

1. A method for producing a desired recombinant polypeptide comprising culturing a mammalian host cell expressing a desired recombinant polypeptide in the presence of a human Growth Hormone (hGH) or modified hGH, wherein expression of the hGH or modified hGH enhances survival and/or cell density and/or cell viability of the mammalian host cell expressing the desired recombinant polypeptide.