| 发明名称 |
ENZYME STALLING METHOD |
| 摘要 |
The invention relates to new methods of moving helicases past spacers on polynucleotides and controlling the loading of helicases on polynucleotides. The invention also relates to new methods of characterising target polynucleotides using helicases. |
| 申请公布号 |
US2016010147(A1) |
申请公布日期 |
2016.01.14 |
| 申请号 |
US201414773164 |
申请日期 |
2014.01.22 |
| 申请人 |
OXFORD NANOPORE TECHNOLOGIES LIMITED |
发明人 |
HERON Andrew John;ALVES David Antoni;CLARKE James Anthony;CRAWFORD Marion Louise;GARALDE Daniel Ryan;HALL Graham;TURNER Daniel John;WHITE James |
| 分类号 |
C12Q1/68;C12N9/14;C12N13/00 |
主分类号 |
C12Q1/68 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method of moving one or more stalled helicases past one or more spacers in a polynucleotide, comprising contacting (a) the one or more stalled helicases and the polynucleotide with a transmembrane pore and (b) applying a potential across the pore and thereby moving the one or more helicases past the one or more spacers on the polynucleotide. |
| 地址 |
Oxford, Oxfordshire GB |
