Methods and compositions to modify the immunogenicity of a vascularized organ or tissue

摘要

Disclosed herein is an RGD-enriched solubilized extracellular matrix composition derived from endothelial cell culture that can be used to modify the immunogenicity or thrombogenicity of an organ intended for transplant. The RGD-enriched solubilized extracellular matrix composition is applied to the lumen of the vasculature of the organ, thereby placing a barrier between the antigens on the lumenal surfaces of the transplanted organ and the blood of the recipient.

主权项

1. An Arg-Gly-Asp (RGD)-enriched solubilized extracellular matrix isolated from cultured corneal endothelial cells (CEC) or human umbilical vascular endothelial cells (HUVECs), said matrix comprising protein content in the amount of 1-5 mg/ml of total ECM and having an RGD absorbance at 450 nm of 0.3 to 0.7, and a fragment size of less than 2 microns.