| 主权项 |
1. A method of analyzing a sample from a patient for cell-free DNA associated with prostate cancer to calculate a numeric index of tumor-associated copy number variability , the method comprising:
isolating cell-free DNA from a blood, serum, or plasma sample from the patient; sequencing cell-free DNA molecules; for each of a plurality of circulating cell-free DNA molecules, unambiguously assigning cell-free DNA sequences to a genomic region; determining the normalized copy numbers of the plurality of cell-free DNA molecules; comparing the normalized copy numbers to a reference value; summing all regions that have a normalized copy number greater than that of 95% of the reference value; and summing all regions that have a normalized copy number less than 5% of the reference value, to obtain a numeric index of tumor-associate copy number variability. |
