METHODS OF MULTIPLEXING DNA SENSORS AND LOCALIZING DNA SENSOR

摘要

The present disclosure relates to a method of multiplexing DNA sensors and optionally measuring pH in cell, a method of localizing DNA sensor in Golgi Network of scFv-Furin expressing cell and a method of identifying optimal location of fluorophore pair on the DNA sensor for multiplexing DNA sensors. The DNA sensors of the present disclosure follow independent cellular pathways and do not interact with or compromise functionality of another DNA sensor.

主权项

1. A method of multiplexing DNA sensors and optionally measuring pH in cell, wherein said DNA sensor comprises Nucleic Acid Assembly and fluorophore, optionally along with protein, said method comprising acts of:
a) adding the DNA sensors to the cell in labelling media for cellular uptake, to obtain a cell with the DNA sensors; b) incubating the cell obtained in step a) in the labelling media for multiplexing the DNA sensors, wherein the DNA sensors are engineered to follow specific cellular pathways within the cell; and c) mapping the multiplexed DNA sensors within the cell at time intervals, optionally determining D/A ratio and obtaining calibration curve for measuring pH in the cell.