发明名称 |
METHODS AND COMPOSITIONS FOR PREPARATION OF NUCLEIC ACIDS |
摘要 |
A method for isolating genomic DNA (gDNA) from a biological material. In some embodiments, the method includes (a) contacting a sample that contains gDNA with a solution of hydroxide and a detergent under conditions and for a time sufficient to degrade a cell wall, a cell membrane, a nuclear membrane, a nucleoprotein, or combinations thereof and/or to denature the gDNA; (b) mixing into the solution resulting from step (a) a solution characterized by high salt and sufficient buffering capacity to reduce the pH of the solution to less than 10, thereby producing a neutralized solution; (c) centrifuging the sample at a speed and length of time sufficient to clarify the preparation; and (d) removing insoluble material from the neutralized and clarified preparation, whereby a solution of gDNA is produced. Also provided are method for performing a quantitative polymerase chain reaction (qPCR) of a gDNA sample, methods for performing genotyping or other molecular marker analysis of a gDNA sample, and methods for determining a haplotype of a cell. |
申请公布号 |
EP2961264(A1) |
申请公布日期 |
2016.01.06 |
申请号 |
EP20140756408 |
申请日期 |
2014.02.10 |
申请人 |
SYNGENTA PARTICIPATIONS AG |
发明人 |
JI, YANSHAN;WANG, WENLING;HUANG, ER-TE JAMIE;MOORE, KAREN ELIZABETH;WHITE, KIMBERLY ANN |
分类号 |
A01H5/00;C12N1/06;C12N15/10;C12P19/34;C12Q1/68 |
主分类号 |
A01H5/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|