Complementing cell lines

摘要

A packaging cell line that complements recombinant adenoviruses based on serotypes from subgroup B, preferably adenovirus type 35. The cell line may be derived from primary, diploid human cells that are transformed by adenovirus E1 sequences either operatively linked on one DNA molecule or located on two separate DNA molecules, the sequences being operatively linked to regulatory sequences enabling transcription and translation of encoded proteins. Also disclosed is a cell line derived from PER.C6® that expresses functional Ad35 E1B sequences. The Ad35-E1B sequences are driven by the E1B promoter or a heterologous promoter and terminated by a heterologous poly-adenylation signal. The cell lines are useful for producing recombinant adenoviruses designed for gene therapy and vaccination, and can also be used for producing human recombinant therapeutic proteins such as human growth factors and human antibodies. Also, the cell lines are useful for producing human viruses other than adenovirus such as influenza virus, herpes simplex virus, rotavirus, and measles virus.

主权项

1. An adenovirus packaging cell line permissive for replication of an E1A/E1B deficient adenovirus vector, wherein the cell line comprises an adenovirus E1A coding sequence operably linked to a first promoter and an adenovirus E1B coding sequence operably linked to a second promoter, separate from the first promoter, wherein both the first and second promoters lack substantial sequence identity with a native adenovirus E1A or E1B promoter, and wherein the adenovirus E1A coding sequence and the adenovirus E1B coding sequence are stably integrated into the cell line.