MULTIPLEX DNA NANOPARTICLE PROTEIN ASSAY AND QUANTITATION

摘要

Disclosed are DNA nanoparticles (DeNAno) comprising rolling circle replicated circular oligonucleotides. Also disclosed are methods of multiplexed protein assay and quantitation, the method comprising the steps of creating a DeNAno particle by rolling circle replication of one or more circular oligonucleotide templates; creating one or more libraries of DeNAno particles by using templates with random sequences; selecting the DeNAno particles that bind specifically to magnetic beads coated with antibodies against a target of interest; synthesizing bead specific DeNAno particles and using the same to mask the beads; pooling DeNAno masked beads against multiple target antigens, and adding the pooled DeNAno masked beads to an analyte containing sample, wherein the analytes recognized by their cognate antibodies displace the masking DeNAno particles in a concentration dependent fashion; removing the beads from the sample; and amplifying and sequencing the displaced DeNAno particles. Further disclosed are kits comprising: a plurality of DeNAno particles; instructions for their use; and analysis algorithms.