Process for the production of patches or derssings of autologous skin through cultivation of autologous keratinocytes and fibroblasts with autologous serum for the generation of skin

摘要

The process in general is based on taking a sample of skin and a sample of blood from the patient and based on these two elements skin is cultured, being placed on a collagen patch to produce a dressing which is subsequently placed on the patient requiring same.

主权项

1. A process for generating a skin layer patch or a dressing for a patient in need thereof, characterized in that it comprises the steps of:
obtaining, from a patient requiring a skin layer patch or dressing, an autologous skin sample and a blood sample; digestion of the autologous skin sample for 18 hours; obtaining autologous serum from the patient's blood sample, separating the digested autologous skin into two layers, a superficial epidermis layer and a deep layer of dermis; culturing and replication of keratinocytes in the autologous serum for at least 72 h, wherein the keratinocytes are obtained from the superficial layer of the digested autologous skin; culturing and replication of fibroblasts obtained from the deep layer of the digested autologous skin; seeding the cultured fibroblasts on a collagen mesh; and seeding the cultured keratinocytes on the fibroblast-seeded collagen mesh and then culturing the keratinocyte-seeded collagen mesh for 24 h to obtain the skin layer patch or dressing for the patient.