| 发明名称 | REIC-expressing adenovirus vector | ||
| 摘要 | An objective of the present invention is to provide an adenovirus vector expressing a REIC/Dkk-3 protein at a high level and containing a DNA construct for expression of REIC/Dkk-3 DNA, wherein the DNA construct is prepared by ligating, from the 5′ terminal side, (i) a CMV promoter,(ii) REIC/Dkk-3 DNA,(iii) a polyA addition sequence, and(iv) enhancers prepared by linking an hTERT (Telomerase Reverse Transcriptase) enhancer, an SV40 enhancer, and a CMV enhancer in this order. | ||
| 申请公布号 | US9222107(B2) | 申请公布日期 | 2015.12.29 |
| 申请号 | US201214119772 | 申请日期 | 2012.05.25 |
| 申请人 | National University Corporation Okayama University;Momotaro-Gene Inc. | 发明人 | Kumon Hiromi;Huh Namho;Sakaguchi Masakiyo;Watanabe Masami |
| 分类号 | C12N15/86;A61K48/00;A61K38/00;C07K14/47;A61K38/17;A61K35/761 | 主分类号 | C12N15/86 |
| 代理机构 | Foley & Lardner LLP | 代理人 | Foley & Lardner LLP |
| 主权项 | 1. A DNA construct for expression of REIC/Dkk-3 DNA, which is prepared by ligating the following (i) to (iv) in this order, from the 5′ terminal side: (i) a CMV promoter; (ii) the following REIC/Dkk-3 DNA: (a) DNA comprising the nucleotide sequence shown in SEQ ID NO: 1, or(b) a polynucleotide consisting of SEQ ID NO: 3 or SEQ ID NO: 4; (iii) a polyA addition sequence; and (iv) enhancers prepared by linking an hTERT (Telomerase Reverse Transcriptase) enhancer, an SV40 enhancer, and a CMV enhancer in this order. | ||
| 地址 | Okayama JP | ||