MEMBRANE TRANSLOCATION ASSAY FOR SIRNA

摘要

Disclosed herein is an assay that directly measures the translocation of an siRNA across a lipid bilayer of a liposome. In one embodiment, a process for determining the amount of siRNA translocated across a liposome membrane comprises (a) having a synthetic liposome vesicle with protease chymotrypsin encapsulated inside; (b) having a chymotrypsin inhibitor in bulk solution outside the liposome vesicle; (c) introducing an siRNA to the bulk solution; and (d) measuring the amount of the siRNA in the liposome.