发明名称 |
ENHANCING EFFICIENCY OF RETROVIRAL TRANSDUCTION OF HOST CELLS |
摘要 |
The present invention provides methods for enhancing transduction efficiency of a viral vector into a host cell such as an unstimulated stem cell. The methods involve transducing the host cell with the vector in the presence of an SAMHD1 inhibitor (e.g., a Vpx protein), and an inhibitor of mTOR complexes (e.g., rapamycin or analog compound thereof). Also provided in the invention are kits or pharmaceutical combinations for delivering a therapeutic agent into a target cell with enhanced targeting frequency and payload delivery. The kits or pharmaceutical combinations typically contain a viral vector encoding the therapeutic agent, an SAMHD1 inhibitor or a polynucleotide encoding the SAMHD1 inhibitor, and an inhibitor of mTOR complexes. |
申请公布号 |
WO2015027142(A3) |
申请公布日期 |
2015.12.23 |
申请号 |
WO2014US52258 |
申请日期 |
2014.08.22 |
申请人 |
THE SCRIPPS RESEARCH INSTITUTE;TORBETT, BRUCE;WANG, CATHY, X. |
发明人 |
TORBETT, BRUCE;WANG, CATHY, X. |
分类号 |
C12N15/00;C12N5/02 |
主分类号 |
C12N15/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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