GLUCOOLIGASACCHARIDES, CONTAINING (ALPHA 1-4) AND (ALPHA-1-6) GLYCOSIDIC LINKAGES, THEIR APPLICATION AND METHODS OF THEIR OBTAINING

摘要

FIELD: biotechnologies.SUBSTANCE: method of obtaining of the mix of glucooligasaccharides containing two or more series (?1?6) glycosidic linkages and two or more series (?1?4) glycosidic linkages is offered. Poly-and/or oligosaccharide comprising on non-restorable end at least two (?-1?4)-linked units of D-glucose, are forced to contact with the enzyme ?-glucanotransferase enzyme. The named enzyme is GTFB type of enzyme and splits (?1?4) and creates new (?1?4) and (?1?6) glycoside links. The released linear glucooligasaccharides and their mixes obtained by the named method, their use as fibre-prebiotic in food composition are offered. Also the genetically modified GTFA-type glucan sucrase enzyme including at least two mutations indicated in the invention formula and with GTFB-type activity is also offered. The named modified enzyme splits (?1?4) and creates new (?1?4) and (?1?6) glycosidic linkages, using as a substratum the poly-and/or the oligosaccharides containing (?1?4)-linked units of D-glucose on its non-restoring end.EFFECT: inventions allow to obtain partially indigestible derivatives of starch.19 cl, 2 tbl, 8 dwg