MULTIPLEX NUCLEIC ACID AMPLIFICATION

摘要

In some embodiments, the disclosure relates generally to compositions, comprising a single reaction mixture containing a plurality of different populations of discrete supports, and a plurality of different populations of target nucleic acids. The single reaction mixture can contain a first population of beads; a second population of beads; a first population of target nucleic acids, where at least two different target nucleic acids in the first population of target nucleic acids can bind to a bead in the first population of beads; and a second population of target nucleic acids, where at least two different target nucleic acids in the second population of target nucleic acids can bind to a bead in the second population of beads. The single reaction mixture can be employed to monoclonally amplify the first target nucleic acids on the first beads, and monoclonally amplify the second target nucleic acids on the second beads.

主权项

1. A method of nucleic acid synthesis, comprising:
a) providing a single reaction mixture containing a plurality of beads of a first type, the beads of the first type including first capture primers; a plurality of beads of a second type, the beads of the second type including second capture primers, wherein the first and second capture primers are different; a first population of target nucleic acids, wherein the first population includes at least one target nucleic acid of a first type that binds to the first capture primers; a second population of target nucleic acids, wherein the second population includes at least one target nucleic acid of a second type that binds to the second capture primers; b) forming a first amplified population of nucleic acids by amplifying one or more target nucleic acids of the first population, wherein the first amplified population is attached to one or more beads of the first type; and c) forming a second amplified population of nucleic acids by amplifying one or more target sequences from the second population of target nucleic acids, wherein the second amplified population is attached to one or more beads of the second type.