发明名称 |
Purification of Organic Compounds Using Surrogate Stationary Phases on Reversed Phase Columns |
摘要 |
There are only two ways to increase the amount of sample that can be purified by preparative reversed phase high performance liquid chromatography (Prep-RP-HPLC) in a single run: (1) The traditional approach is to use a bigger column (greater amount of stationary phase); and (2) Use displacement chromatography which uses the stationary phase more effectively. This invention describes a unique Prep-RP-HPLC technique that uses a C-18/C-8 derivatized silica coated with a hydrophobic quaternary ammonium salt or quaternary phosphonium salt to result in 7 to 12 fold increase in sample loading (of the crude mixture of organic compounds including synthetic crude peptides) in contrast to the conventional Prep-RP-HPLC technique. This increase in sample loading capacity and output is due to the additional surrogate stationary phase characteristic of the C-18/C8 bound quaternary salt. The quaternary surfactant is bound to the C-18/C-8 chains and silanols of the stationary phase. |
申请公布号 |
US2015360146(A1) |
申请公布日期 |
2015.12.17 |
申请号 |
US201314763831 |
申请日期 |
2013.07.01 |
申请人 |
NEULAND HEALTH SCIENCES PRIVATE LIMITED |
发明人 |
ANWER Mohammed Khalid |
分类号 |
B01D15/32;B01D15/42;B01D15/36;C07K7/06;C07K1/20 |
主分类号 |
B01D15/32 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
Hyderabad IN |