System Comprising a Mass Spectrometer coupled to a FAIMS Apparatus and Methods of Operation

摘要

A method of operating a system comprising a chromatograph and a mass spectrometer comprises: (a) providing an abundance threshold and a list comprising respective entries for precursor ion species of interest comprising respective precursor-ion m/z ratios; (b) transmitting a first sample fraction portion comprising a plurality of sample-fraction ion species through an ion mobility spectrometer operated in non-dispersive mode to the mass spectrometer; (c) detecting a respective abundance at each of a plurality of sample-fraction m/z ratios; and (d) upon detection of an above-threshold ion abundance at an m/z-ratio corresponding to a first precursor ion species of interest: (d1) inletting a second sample fraction portion into the ion mobility spectrometer operated in dispersive mode such that ions of the first precursor-ion species are preferentially transmitted therethrough; (d2) fragmenting the preferentially-transmitted ions so as to generate product ion species; and (d3) detecting the product ion species.

主权项

1. A method of operating a system comprising a chromatograph operable to separate sample solutions into fractions, an ion source operable to ionize components of the fractions and a mass spectrometer operable to analyze and detect the ions, the method comprising:
(a) providing an abundance threshold value and a list comprising respective entries for each of two or more precursor ion species of interest comprising respective precursor-ion m/z ratios; (b) transmitting a first portion of a sample fraction comprising a plurality of sample-fraction ion species through an ion mobility spectrometer to the mass spectrometer, wherein the ion mobility spectrometer is operated in a non-dispersive mode; (c) detecting a respective ion abundance at each of a plurality of sample-fraction m/z ratios using the mass spectrometer; and (d) upon detection of an above-threshold ion abundance at a sample-fraction m/z-ratio corresponding to a first one of the precursor ion species of interest:
(d1) inletting a second portion of the sample fraction into the ion mobility spectrometer, wherein the ion mobility spectrometer is operated in dispersive mode such that ions of the first precursor-ion species are preferentially transmitted through the ion mobility spectrometer to the mass spectrometer;(d2) fragmenting the preferentially-transmitted ions so as to generate a first set of product ion species; and(d3) detecting the first set of product ion species using the mass spectrometer.