The present invention relates to methods of imaging template hybridisation for estimating cluster numbers prior to solid phase amplification and sequencing. More particularly, an initial round of imaging is carried out at the single molecule template hybridisation stage which allows a general estimation of cluster numbers prior to clusters being formed. Amplification of the signal allows single molecule imaging to be carried out using standard sequencing imaging apparatus.
申请公布号
CA2951005(A1)
申请公布日期
2015.12.17
申请号
CA20152951005
申请日期
2015.06.11
申请人
ILLUMINA CAMBRIDGE LIMITED
发明人
RASOLONJATOVO, ISABELLE MARIE JULIA;BOUTELL, JONATHAN MARK;SMITH, VINCENT PETER;RIGATTI, ROBERTO