摘要 |
The present invention relates to a method for preparing ( Z )-6,7-dihydro-1 H -azepin-2(5 H )-one comprising removing the ±-amino group of ±-amino-µ-caprolactam, wherein the removal is catalysed by a biocatalyst. The invention further relates to a method for preparing caprolactam from ( Z )-6,7-dihydro-1 H -azepin-2(5 H )-one. The invention further relates to a host cell comprising at least one recombinant vector comprising a nucleic acid sequence encoding a biocatalyst with L-±-amino-µ-caprolactam ammonia lyase activity. |