| 摘要 |
The present invention provides methods to make soluble or membrane-anchored metabolic regulators for use in prokaryotes or eukaryotes. The present invention provides methods of expressing novel protein constructs that bind to specific metabolites to control their availability in the cell. The invention utilizes bacterial periplasmic binding proteins, or domains from prokaryotic and eukaryotic proteins that are functionally similar to the bacterial periplasmic binding proteins, fused together and/or fused to a peptide that encodes a transmembrane domain. Changes in metabolite availability will result in altered metabolism or receptor activity to improve growth, yield, crop quality, or tolerance to biotic and abiotic stress.
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