| 摘要 |
A method of improving signal-to-noise performance of a probe. A probe or set of probes is provided for hybridization with a sample. The probe or set of probes is hybridized with the sample at a first hybridization stringency over a first hybridization time period. Following the hybridization over the first hybridization time period, the probe or set of probes is hybridized with the sample at a second hybridization stringency over a second hybridization time period. A method of improving signal-to-noise performance of probes on an array includes providing a sample containing sequences that provide perfect complementary matches to sequences contained on at least some of said probes; and hybridizing the probes with the sample, while cycling the stringency of hybridization conditions during the hybridization.
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