RECOMBINASE POLYMERASE AMPLIFICATION REAGENTS AND KITS
摘要
This disclosure describes kits, reagents and methods for Recombinase Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed kits, reagents and methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods.
申请公布号
WO2010141940(A1)
申请公布日期
2010.12.09
申请号
WO2010US37611
申请日期
2010.06.07
申请人
ALERE SAN DIEGO, INC.;PIEPENBURG, OLAF;ARMES, NIALL, A.